Rockefeller University Research Assistant | Laboratory of Neurophysiology and Behavior in New York, New York
The Laboratory of Neurophysiology and Behavior takes advantage of the relatively simple nervous system of the fruit fly Drosophila to examine how flexibility is engineered into the nervous system to give rise to adaptive behaviors, at the level of both individuals and species. We examine how learning rapidly shapes neural pathways to allow each member of a species to respond appropriately to their unique experience of the world. We also consider how neural circuits are adapted over longer evolutionary time scales to give rise to species-specific behaviors. Our lab employs a multidisciplinary approach, from biochemistry to behavior, with the goal of revealing the neural basis for flexible behavior at high resolution.
We are seeking an experienced and talented molecular biologist who will contribute to ongoing work studying how neural circuits evolve across Drosophila species to mediate species-specific behaviors. The work will include designing and implementing different genome editing strategies, such as PiggyBac, PhiC31 and CRISPR-Cas9, to generate novel genetic reagents in non-model Drosophila species.
Primary responsibilities will include:
Designing and constructing targeting vectors
Screening transformants by fluorescent markers and/or genotyping and Drosophila husbandry
Anatomic, functional or behavioral characterization at both the larval and adult stage of generated lines
Being an active contributor to research projects, including in the conceptual design and analysis of experiments, and presentation of data in laboratory meetings.
A Bachelor's degree (or higher) in molecular biology, genetics, or closely related field
Extensive experience in molecular biology including CRISPR-Cas9 genome editing
Experience designing complex cloning strategies and troubleshooting molecular biology experiments
Excellent organizational, communication and time management skills
Experience working with Drosophila and a solid foundation in fly genetics, desirable
Detail-oriented, focused, highly organized and a fast learner
Creative and stay abreast of advanced in molecular biology
Proactive and take ownership of your work
Able to work within a team as well as independently
Able to manage multiple projects concurrently
Excited to contribute to innovative science
Expertise in a subset of the following techniques is highly desirable: DNA/RNA extraction, PCR (conventional, multiplex, touchdown, reverse transcription, genotyping), cloning (restriction enzyme, Gibson assembly, Gateway), CRISPR-Cas9 genome editing, Drosophila genetics, or Drosophila husbandry, IHC and FISH assays.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans.
Compensation Range: Min
Compensation Range: Max
Job Locations US-NY-New York
Position Type Regular Full-Time