Job Information
Rockefeller University Research Assistant | Laboratory of Molecular Biology in New York, New York
Organization Overview
Our research is aimed at the identification of molecular mechanisms that control development and dysfunction of the mammalian cerebellum. We have chosen to study the cerebellum because it is a complex and highly stereotyped structure in which major pattern formation and functional organization occur postnatally. During the past century,classical neuroanatomical studies have very precisely described the complex events that must occur for formation of this brain structure,providing what might be considered as the most detailed information available concerning the development of a given brain region. An extensive literature has documented the importance of cell-cell interactions in the development and maintenance of normal cerebellar architecture,although the mechanisms mediating these interactions remain unknown. Identification of these mechanisms,and analysis of their functions throughout the central nervous system (CNS),are major priorities of the Heintz laboratory.
Overview
The goal of this project is to identify novel strategies to delay onset and/or slow progression of Huntingdon's disease. The Research Assistant will be responsible for the preparation of human brain samples for next generation sequencing.
Responsibilities
Specific tasks include:
Subcellular fractionation
Isolation of cell components with antibodies or DNA probes (via FACs)
RNA extraction
cDNA preparation
PCR, gel electrophoresis and library preparation
Preparing genetic material for trinucleotide repeat sizing
Qualifications
Requirements:
Bachelor's degree
Good computer skills and familiarity with NCBI database tools.
Good communication skills
Ability to keep clear accurate records
Preferred:
Previous experience with RNA isolation and in situ hybridization
Familiarity with established profiling assays such as ATAC-Seq, bisulfite-Seq and Ox-bisulfite-Seq to elucidate the epigenetic properties of the DNA
The Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.
The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller University's good faith and reasonable estimate of the range of possible compensation at the time of posting.
Compensation Range: Min
USD $48,000.00/Yr.
Compensation Range: Max
USD $52,000.00/Yr.
Job Locations US-NY-New York
Category Science
Position Type Regular Full-Time